Welcome to AliGater’s documentation!¶
- Getting started
- Example Strategies
- Introduction
- Loading an example .fcs file
- Gating PBMCs using shortest path algorithms.
- Gating singlets using principal component based clustering
- Separating CD3 positives using local minima (valleySeek)
- Separating CD4 / CD8 using a quad gate
- Gating T-reg cells using density delimitation and bezier curves
- gating CD194 positives (valleySeek)
- T-helper subsets (valleySeek and densityDelimitation)
- CXCR5 positives (valleySeek and densityDelimitation)
- Follicular T-helper cells (densityDelimitation)
- Follicular T-helper subsets (quadGate)
- FMO based thresholds for HLA-DR positivity (gateThreshold)
- CD8 positive subsets (valleySeek and quadGate)
- Some more FMO based thresholds for HLA-DR positivity (gateThreshold)
- Classes
- Methods